Evans Blue Results

Lab Tools

Protocol setup

Groups, mice and assay settings

0 mice

96-well plates Brain extraction volume (µL) Serum dilution factor

Protocol standards50–2000 ng/mL8 concentrations · 2 wells each

Reference group (optional)

Paste Mouse ID + 4 Tube weights (g)

Group	Mouse ID	Ipsi empty Tube (g)	Ipsi Tube + brain (g)	Ipsi tissue (g)	Contra empty Tube (g)	Contra Tube + brain (g)	Contra tissue (g)

620 / 680 nm fluorescence

0 assigned

Assignment Concentration Group Mouse Specimen

Drag across wells to select.

Analysis output

Each used plate is fitted independently with an intercept.

Plate fits

Plate	Slope	Intercept	R²	Range (ng/mL)

Sample results

Sample	Group	Specimen	Wells	n	Fluorescence values	CV%	Curve conc. (ng/mL)	Tissue weight (g)	Final result	Normalized	Range