OurNest
Lab Tools
Toggle navigation
Home
Planner
C57
Transgenic
OnTarget
Lab Tools
Lab Tools
Overview
WB Quick
BCA Assay
RT-qPCR
Lab Tools
/ Gene expression
RT-qPCR Quick Analysis
ΔCt → 2
−ΔΔCt
→ reference mean = 1
1
Experiment setup
Genes, groups and plates
0 / 96 wells
Extra replicate wells
96-well plates
Ct QC cutoff
Genes
+ Add gene
Groups and Sample IDs
+ Add group
2
Plate design
Assign samples and genes
0 assigned
Auto layout
Clear layout
Drag selection
Rectangle
Continuous (A1 → H12)
Groups
Samples
Genes
Fill selection
Clear selected
Drag across wells to select.
3
Ct values
Paste and review each plate
Click a heatmap well to exclude
Analyze RT-qPCR
Analysis output
Normalized gene expression
Download CSV
Group summary
Gene
Group
n
Mean
SD
SEM
Sample results
Gene
Group
Sample
Target Ct
Control Ct
ΔCt
ΔΔCt
2
−ΔΔCt
Normalized
Technical replicate QC
Group
Sample
Gene
Valid / Total
Excluded
Undetermined
Mean Ct
SD
Max difference
QC