Seahorse XLSX
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Lab Tools / Cellular metabolism
Seahorse Mito Stress Analysis
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Plate review
0 selected
Samples, normalization and QC
Protein normalization
0 entered
Paste total protein by well
Blank Background wells are allowed. Values must be greater than zero.
Background QC
QC only
Inspect Raw background signals
Background slope plate map
Click a Background well to exclude or restore it in the QC preview.
Background well statistics and exclusion log
| Well | Mean signal | Slope / tick | R² | Difference from median | QC | Preview status |
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Scale 1.0 preserves the Wave Rate result. Lower values weaken the estimated Background subtraction; use the linked OCR preview to check the direction and size of the change. Exclusions and scaling feed an experimental in-app re-analysis; original Wave Rate values remain preserved for traceability.
Click a well to exclude or restore it. Drag across wells to select them for editing; use Ctrl/Shift-click to select one well without excluding it.
Analysis output
Cell Mito Stress Test
OCR + ECAR kinetics
Metabolic traces
Choose a sample or click a curve point to highlight the same well in both charts.
Merged groups
Mitochondrial parameters
Group summary
| Group | Metric | n | Mean | SD | SEM |
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Sample results
| Sample | Group | Source wells | Technical wells | QC | Basal | ATP-linked | Proton leak | Maximal | Spare | Non-mito | Coupling % |
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Well QC and calculation details
| File | Well | Sample | Group | Baseline | Oligomycin | FCCP | Rot/AA | QC flags |
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